When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.
Get tips on using Silencer® Select GLO-1 siRNA to perform siRNA / miRNA gene silencing Human - Primary Human Aortic Endothelial Cells GLO-1 Lipid
Get tips on using CA125 Monoclonal Antibody (Ov185:1) to perform Immunohistochemistry Human - CA125
Get tips on using Aspergillus antibody | WF-AF-1 to perform Immunohistochemistry Mouse - Aspergillus
Get tips on using HSP60 monoclonal antibody (LK-1) to perform Western blotting Hsp60
Get tips on using Human Ubiquitin/Ubiquitin+1 Antibody to perform Western blotting Ubiquitin
Get tips on using Human Syndecan-1 DuoSet ELISA to perform ELISA Human - SDC1
Get tips on using Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) to perform Flow cytometry Anti-bodies Mouse - CD16/CD32
Get tips on using TriDye™ 1 kb Plus DNA Ladder to perform DNA Ladder 1 kb
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment