Site Directed Mutagenesis (SDM) Human Point mutation IMR-32

Get tips on using Human LBP PicoKine™ ELISA Kit to perform ELISA Human - LBP

Get tips on using Human Fibronectin PicoKine™ ELISA Kit to perform ELISA Human - Fibronectin

Get tips on using Human FABP2/I-FABP DuoSet ELISA to perform ELISA Human - FABP2

Get tips on using Human Total EGFR DuoSet IC ELISA to perform ELISA Human - EGFR

Get tips on using EGFR (Full-length) Human ELISA Kit to perform ELISA Human - EGFR

Get tips on using Human Decorin ELISA Kit (DCN) (ab99998) to perform ELISA Human - Decorin

Get tips on using Human BDNF PicoKine™ ELISA Kit to perform ELISA Human - BDNF

Get tips on using Adiponectin Human ELISA, High Sensitivity (Sandwich) to perform ELISA Human - Adiponectin

Get tips on using VWF (Human) ELISA Kit to perform ELISA Mouse - vWF-A2

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.