siRNA / miRNA gene silencing Human CRL-5915

Get tips on using MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), FITC, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - MHCII

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), eFluor 450, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - MHCII

The most widely used method for protein quantification is by spectrophotometry. The concentration of the protein in the samples is measured at an absorbance of 280 nm. The absorbance of the sample protein is then plotted against a standard curve. This method allows for total protein quantification in a sample (cell and tissue extracts). Before analysing the concentration of protein in the sample, it is important to choose the right test method.  For high protein concentration samples (above 5 - 160 mg/ml) the best method is to use the Biuret test. For low concentrations samples (between 1 - 2000µg/ml) the best methods are Lowry assay, BCA assay, Bradford assay and coomassie blue (for exact sensitivity of the test kits you use, refer to manufacturer's protocol). If the samples contain detergents like Triton X-100 then BCA assay is the best choice. For samples that have proteins larger than 3 KDa in size Bradford assay is the best choice. Each method has advantages and disadvantages, plan your analysis considering your sample characteristics.

Get tips on using Trichloroacetic acid to perform Protein isolation Bacteria - Clostridium difficile

Get tips on using QIAGEN Plasmid Kits to perform Plasmid Isolation Enterobacter cloacae

Get tips on using Laemmli Lysis-buffer to perform Protein isolation Bacteria - Clostridium difficile

Get tips on using NucleoSpin® Plasmid EasyPure to perform Plasmid Isolation Clostridium difficile

Get tips on using TRIzol Reagent to perform RNA isolation / purification Bacteria - Gram positive Clostridum botulinum

Get tips on using TRIzol Reagent to perform RNA isolation / purification Bacteria - Gram positive Clostridium tetani