DNA Damage Assay

Get tips on using QuantiPro™ BCA Assay Kit to perform Protein quantification Colorimetric method

Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Mouse - Cardiac fibroblasts

Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Human - SMMC-7721

Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Human - HEK 293

Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Human - SH-SY5Y

Get tips on using RIP-Assay Kit for microRNA to perform ChIP Human - MCF-7

Get tips on using Qubit RNA HS Assay Kit to perform RNA quantification Fuorimetric - HEK293

Get tips on using Qubit RNA HS Assay Kit to perform RNA quantification Fuorimetric - HeLa

Get tips on using cobas® DNA Sample Preparation Kit to perform AAA for reviews

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.