Protein expression and purification

Get tips on using pTRAkc-ERH/pRIC 3.0 to perform Protein Expression Prokaryotic cells - A. tumefaciens BFDV cp

Get tips on using EZQ™ Protein Quantitation Kit to perform Protein quantification Fluorimetric method

Get tips on using Qubit™ Protein Assay Kit to perform Protein quantification Fluorimetric method

Get tips on using NanoOrange™ Protein Quantitation Kit to perform Protein quantification Fluorimetric method

Get tips on using pVL1392-RIP1 8-322-His to perform Protein Expression Eukaryotic cells - S. frugiperda RIP1

Get tips on using pIVEX-GAA-omega-PHACTR1-H to perform Protein Expression Eukaryotic cells - N. benthamiana PHACTR1

Get tips on using pHR-CMV-TetO2-VSV-G to perform Protein Expression Eukaryotic cells - HEK293 VSV-G

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using Qproteome Bacterial Protein Prep Kit to perform Protein isolation Bacteria - Bacillus subtilis