Stem cell culture media

Get tips on using Gibco™ DMEM/F-12, GlutaMAX™ supplement to perform 3D Cell Culture Media hiPSC-derived retinal organoids

Get tips on using Gibco™ DMEM/F-12, GlutaMAX™ supplement to perform 3D Cell Culture Media hESC-derived brain organoids

Get tips on using Gibco™ DMEM, high glucose, GlutaMAX™ Supplement to perform 3D Cell Culture Media Mouse small intestinal organoids

Get tips on using Corning® 500 mL Iscove’s Modification of DMEM to perform 3D Cell Culture Media hiPSC-derived lung organoids

Get tips on using Brain heart infusion bhi agar 20 plates BD Difco BBL to perform Bacterial cell culture media Bordetella bronchiseptica

Get tips on using DMEM/F-12, no phenol red to perform 3D Cell Culture Media Human breast cancer MCF-7 cells-Mammospheres

Get tips on using Corning® 1L DMEM (Dulbecco’s Modified Eagle’s Medium)/F12 50:50 Mix to perform 3D Cell Culture Media Mouse fallopian organoids

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

Get tips on using Gibco™ MEM α, GlutaMAX™ Supplement, no nucleosides to perform 3D Cell Culture Media hiPSC-derived cardiac organoids