Western blot LC3B Rabbit

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ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Proteins ELISA Mouse Chitinase-3-Like Protein-1 (CHI3L1) or YKL-40

Proteins Immunohistochemistry Mouse LC3

Get tips on using Anti-LC3 mAb to perform Autophagy assay cell type - N2a

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Get tips on using Anti-LC3 mAb to perform Autophagy assay cell type - C6

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Get tips on using Anti-LC3 pAb to perform Autophagy assay cell type - NRK

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Get tips on using Anti-LC3 pAb to perform Autophagy assay cell type - U251MG

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Get tips on using Anti-LC3 pAb to perform Autophagy assay cell type - U87MG

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Get tips on using Anti-LC3 Antibody to perform Autophagy assay cell type - MCF7

Products Sigma-Aldrich Anti-LC3 Antibody

Get tips on using Anti-LC3 pAb to perform Autophagy assay cell type - A549

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Get tips on using Anti-LC3 pAb to perform Autophagy assay cell type - HEK 293

Products MBL international corporation Anti-LC3 pAb

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