Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - human breast tissue
Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - human lung tissue
Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - human placental tissue
Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - human brain tissue
Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - human aorta tissue
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
Get tips on using Gibco™Advanced DMEM/F-12 to perform 3D Cell Culture Media Mouse small intestinal organoids
Get tips on using GenElute™ Mammalian Total RNA Miniprep Kit to perform RNA isolation / purification Tissue - Rat Ovaries
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - human breast tissue
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - human lung tissue
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