Immunofluorscence  53BP1 [H-300] Rabbit Human

Get tips on using PerCP-Cy™5.5 Mouse Anti-Human CD19 to perform Flow cytometry Anti-bodies Human - CD19

Get tips on using APC-Cy™7 Mouse Anti-Human CD19 to perform Flow cytometry Anti-bodies Human - CD19

Get tips on using APC-Cy™7 Mouse Anti-Human CD3 to perform Flow cytometry Anti-bodies Human - CD3

Get tips on using Brilliant Violet 570™ anti-human CD27 Antibody to perform Flow cytometry Anti-bodies Human - CD27

Get tips on using Brilliant Violet 605™ anti-human CD69 Antibody to perform Flow cytometry Anti-bodies Human - CD69

Get tips on using PE/Dazzle™ 594 anti-human CD69 Antibody to perform Flow cytometry Anti-bodies Human - CD69

Get tips on using PerCP/Cyanine5.5 anti-human CD127 (IL-7Rα) Antibody to perform Flow cytometry Anti-bodies Human - CD127

Get tips on using Alexa Fluor® 647 Mouse Anti-Human CD24 to perform Flow cytometry Anti-bodies Human - CD24

Get tips on using Human Serpin E1/PAI-1 Quantikine ELISA Kit to perform ELISA Human - Serpin E1/PAI-1

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.