shRNA gene silencing Human THP-1

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

Get tips on using mirVana™ miRNA Isolation Kit, with phenol to perform RNA isolation / purification Cells - immortalized THP-1

Get tips on using RealTime-Glo™ MT Cell Viability Assay to perform Live / Dead assay mammalian cells - THP-1

Get tips on using ADAMTS13 siRNA to perform siRNA / miRNA gene silencing Human - HUVEC ADAMTS-13

Get tips on using siRNA ICAM1 to perform siRNA / miRNA gene silencing Human - HT-1080 ICAM1

Get tips on using Stealth siRNA_GATA2 to perform siRNA / miRNA gene silencing Human - LAD2 GATA2

Get tips on using RAB8A siRNA to perform siRNA / miRNA gene silencing Human - HEK293 Rab8a

Get tips on using KRAS siRNA to perform siRNA / miRNA gene silencing Human - A431 KRAS

Get tips on using STEAP1 siRNA to perform siRNA / miRNA gene silencing Human - LNCap STEAP1