shRNA gene silencing Mouse Prostate cancer cell lines (DU145 and PC3)

Get tips on using in situ Cell Death Detection Kit, POD to perform TUNEL assay cell type - PANC-1 human pancriatic cancer

Get tips on using Direct-zol RNA Kits to perform RNA isolation / purification Cells - immortalized DU145

Get tips on using FastLane Cell cDNA Kit (50) to perform cDNA synthesis Cell lines

Get tips on using RNAprotect Cell Reagent (250 ml) to perform RNA stabilization Cell lines

Get tips on using REPLI-g Single Cell Kit (96) to perform Whole Genome Amplification Cell lines

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using AmpFLSTR™ Identifiler™ PCR Amplification Kit to perform Cell line authentication Endometrial Cancer cell line KLE

Get tips on using AmpFLSTR™ Identifiler™ PCR Amplification Kit to perform Cell line authentication Endometrial Cancer cell line Ishikawa

Get tips on using AmpFLSTR™ Identifiler™ PCR Amplification Kit to perform Cell line authentication Ovarian cancer cell line SKOV3

Get tips on using AmpFLSTR™ NGM™ PCR Amplification Kit to perform Cell line authentication Ovarian cancer cell line SKOV3