siRNA / miRNA gene silencing Mouse Pancreatic Acinar cells

Get tips on using ON-TARGETplus Human PCSK6 (5046) siRNA - Individual to perform siRNA / miRNA gene silencing Human - A253 PACE4

Get tips on using ON-TARGETplus Human FURIN (5045) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - A253 Furin

Get tips on using Hs_TET3_2 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - HCT-116 TET3(TET methylcytosine dioxygenase 3)

Get tips on using siRNA MAPKAPK-2 to perform siRNA / miRNA gene silencing Human - U937 MK2 (MAPK Kinase 2) Viral vectors

Get tips on using siRNA MAPKAPK-2 to perform siRNA / miRNA gene silencing Human - Jurkat MK2 (MAPK Kinase 2) Viral vectors

Get tips on using Silencer® Select Negative Control No 1 siRNA to perform siRNA / miRNA gene silencing Human - siRNA negative control Lipid

Get tips on using ON-TARGETplus Human PPARGC1B siRNA to perform siRNA / miRNA gene silencing Human - MCF-7 PGC-1β/PPARGC1B

Get tips on using 14-3-3ζ siRNA(h) to perform siRNA / miRNA gene silencing Human - Caco-2 14‐3‐3ζ

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

Get tips on using siGENOME Rat Acvr1c (245921) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - INS-1 Alk7/Acvr1c