DNA isolation / purification Cells Primary cells

Generally isolating RNA from Gram-negative bacteria is easy, however keeping your working environment clean and RNase free (use RNase inhibitor) is essential. Some common points to keep in mind: a) Use fresh samples for isolation or store them by freezing in RNA stabilizing buffer until use. b) Choose the bacterial input amounts carefully, to ensure buffer volumes are adequate and not to overload the columns.

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Cells - immortalized SKBR3, MDA-MB231 and MCF7

Get tips on using MinElute 96 UF PCR Purification Kit (24) to perform DNA isolation / purification Plasmid purification

Get tips on using QIAamp BiOstic Bacteremia DNA Kit (50) to perform DNA isolation / purification Fish

Get tips on using AllPrep PowerViral DNA/RNA Kit (50) to perform DNA isolation / purification Viral

Isolating RNA from tissues and paraffin embeded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the intigrity of RNA