Protein expression and purification Insect cells Sf9

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using pMCO-AOXα-An to perform Protein Expression Eukaryotic cells - P. pastoris appA

Get tips on using pPIC9K-anPhyA to perform Protein Expression Eukaryotic cells - P. pastoris Aspergillus niger PhyA

Get tips on using pVRB2B3 to perform Protein Expression Eukaryotic cells - HEK293 hβ-defensin 2/3

Get tips on using pMAPLe3 to perform Protein Expression Prokaryotic cells - E. coli mycobacterial Esx complex

Get tips on using pMAPLe4 to perform Protein Expression Prokaryotic cells - E. coli mycobacterial Esx complex

Get tips on using pCcrtB to perform Protein Expression Prokaryotic cells - E. coli Kocuria gwangalliensis CrtB

Get tips on using pKUC4 to perform Protein Expression Prokaryotic cells - B. licheniformis PphyL′-′xynA fusion

Get tips on using pKUC3 to perform Protein Expression Prokaryotic cells - B. licheniformis PphyL′-′amyE fusion

Get tips on using pFastBac-N to perform Protein Expression Eukaryotic cells - S. frugiperda AvCoV-IBV