Protein Expression Eukaryotic cells S. pastorianus

Get tips on using Tau Protein Ladder, 6 isoforms human to perform Protein Ladder Immunofluorescence

Get tips on using Prestained Protein Ladder (3.5 - 245 kDa) to perform Protein Ladder Prestained

Get tips on using Pierce™ Prestained Protein MW Marker to perform Protein Ladder Prestained

Get tips on using PageRuler™ Prestained NIR Protein Ladder to perform Protein Ladder Prestained

Get tips on using GenElute™ Plasmid Miniprep Kit to perform Plasmid Isolation S. cerevisiae

Get tips on using Gateway™ pDONR™221 Vector to perform Protein expression and purification Yeast - Saccharomyces cerevisiae ΔHSPA5

Get tips on using Micro BCA™ Protein Assay Kit to perform Protein quantification Colorimetric method

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Colorimetric method

Generally it has been difficult to isolate high-quality RNA from yeast because of problems disrupting the cells. Use of enzymes to disrupt cell wall can alter gene expression profiles. Therefore, physical disruption can result in high quality RNA for all downstream processing. Use of DNAse and proteinase K will remove traces of DNA contamination and proteins respectively.

Generally it has been difficult to isolate high-quality RNA from yeast because of problems disrupting the cells. Use of enzymes to disrupt cell wall can alter gene expression profiles. Therefore, physical disruption can result in high quality RNA for all downstream processing. Use of DNAse and proteinase K will remove traces of DNA contamination and proteins respectively.