Protein Expression Prokaryotic cells M. smegmatis

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Generally it has been difficult to isolate high-quality RNA from yeast because of problems disrupting the cells. Use of enzymes to disrupt cell wall can alter gene expression profiles. Therefore, physical disruption can result in high quality RNA for all downstream processing. Use of DNAse and proteinase K will remove traces of DNA contamination and proteins respectively.

RNA RNA isolation / purification Yeast Ustilago maydis

Get tips on using PageRuler™ Unstained Protein Ladder to perform Protein Ladder Unstained

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Get tips on using IRIS9 Plus Prestained Protein Ladder to perform Protein Ladder Prestained

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Get tips on using iBright™ Prestained Protein Ladder to perform Protein Ladder Prestained

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Get tips on using CozyHi™ Prestained Protein Ladder to perform Protein Ladder Prestained

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Get tips on using CozyXL™ Prestained Protein Ladder to perform Protein Ladder Prestained

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Get tips on using Cozy™ Prestained Protein Ladder to perform Protein Ladder Prestained

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Get tips on using pET-21a(+) DNA to perform Protein expression and purification Bacteria - Escherichia coli Prefoldin (PFD)

Products Merck Millipore pET-21a(+) DNA

Get tips on using pQE-80L vector to perform Protein expression and purification Bacteria - Escherichia coli IFABP-Aβ

Products Creative Biogene pQE-80L vector

Get tips on using pPIC9K Pichia Vector to perform Protein expression and purification Yeast - Pichia pastoris N-APP

Products Thermo Fisher Scientific pPIC9K Pichia Vector

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