Protein expression and purification Bacteria Bacillus subtilis

Get tips on using MagMAX™ Total Nucleic Acid Isolation Kit to perform DNA isolation / purification Bacteria - Gram positive Mycobacterium tuberculosis

Get tips on using MagMAX™ Total Nucleic Acid Isolation Kit to perform RNA isolation / purification Bacteria - Gram positive Mycobacterium tuberculosis

Get tips on using TAGZyme pQE Vector Set to perform Protein tag Expression of His-tagged proteins

Get tips on using MagNA Pure Compact Nucleic Acid Isolation Kit I to perform DNA isolation / purification Bacteria - Gram negative Enterobacteriaceae

Get tips on using PowerSoil® DNA isolation - DNeasy PowerSoil Pro Kit to perform DNA isolation / purification Bacteria - Gram negative Enterobacteriaceae

Get tips on using pLsLF-CTB-Fx3Pris to perform Protein Expression Eukaryotic cells - N. benthamiana proinsulin

Get tips on using pLDutr-CTB-Fx3Pris to perform Protein Expression Eukaryotic cells - lettuce leaves proinsulin

Get tips on using pKS2-ST::CBHII to perform Protein Expression Eukaryotic cells - W. anomalus CBHII

I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method?

Get tips on using RIPA Lysis and Extraction Buffer to perform Protein isolation Mammalian cells - HepG2