Immunohistochemistry Alpha smooth muscle Actin Rabbit Mouse

Get tips on using Anti-alpha smooth muscle Actin antibody to perform Immunohistochemistry Alpha smooth muscle Actin - Rabbit Mouse -NA-

Get tips on using anti-alpha-Smooth Muscle Actin mouse monoclonal, ASM-1 to perform Immunohistochemistry Alpha smooth muscle Actin - Mouse -NA- -NA-

Get tips on using alpha-Smooth Muscle Actin Antibody to perform Western blotting Smooth muscle actin

Get tips on using Anti-alpha smooth muscle Actin antibody (ab5694) to perform Immunohistochemistry Mouse - SMA

Get tips on using Recombinant Anti-alpha smooth muscle Actin antibody [E184] (ab32575) to perform Western blotting Smooth muscle actin

Get tips on using Monoclonal Mouse Anti-Human Actin (Smooth Muscle) (Concentrate) Clone 1A4 to perform Immunohistochemistry Mouse - SMA

Get tips on using Smooth Muscle Actin Antibody (B4): sc-53142 to perform Immunohistochemistry Mouse - SMA

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

Get tips on using Smooth Muscle Cell Medium to perform Mammalian cell culture media HAOSMC