Get tips on using N-cadherin Polyclonal Antibody to perform Western blotting N‐cadherin
Get tips on using pFastBac-N to perform Protein Expression Eukaryotic cells - S. frugiperda AvCoV-IBV
Get tips on using N-WASP siRNA (h) to perform siRNA / miRNA gene silencing Human - T47-D N-WASP
Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.
Get tips on using Anti-N-Cadherin Antibody, clone 13A9 to perform Western blotting N‐cadherin
Get tips on using N-cadherin Antibody (8C11): sc-53488 to perform Western blotting N‐cadherin
Get tips on using ATG5 Antibody (N-term) to perform Autophagy assay cell type - RPE-J cells
Get tips on using ATG5 Antibody (N-term) to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)
Get tips on using SnoA/N siRNA (h) to perform siRNA / miRNA gene silencing Human - SW1990 SnoN
Get tips on using Bax antibody [N1N2], N-term to perform Western blotting Bax
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