3D Cell Culture Media Human cancer colon organoids

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Found 2 matching solutions for this experiment

Upstream tips
Defrost the Matrigel in advance on ice or in fridge
Protocol tips
Replace culture medium every 2 days
Downstream tips
After removing the culture medium, RNA can be extracted with Trizol. For immunofluorescence, organoids can be fixed with 4% PFA for 30 min, permeabilized, and blocked for non-specific binding with 3% BSA prior to incubation with primary antibodies.
Gibco™Advanced DMEM/F-12

Thermo Fisher Scientific

Upstream tips
Defrost the Matrigel in advance on ice or in fridge
Protocol tips
Use of 48-well plates
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