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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
| Defrost the growth factor reduced Matrigel in advance, on ice or in fridge |
Seed esophageal organoid units in ∼2 mL per well of growth factor reduced Matrigel, spreading over the bottom of a six-well plate
Culture for 10 days with medium changed every other day
|
Collect organoids for microscopic analysis |
| Upstream tips |
| Defrost the growth factor reduced Matrigel in advance, on ice or in fridge |
| Protocol tips |
Seed esophageal organoid units in ∼2 mL per well of growth factor reduced Matrigel, spreading over the bottom of a six-well plate
Culture for 10 days with medium changed every other day
|
| Downstream tips |
| Collect organoids for microscopic analysis |
| Upstream tips |
Protocol tips |
Downstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
Seed 2000 cells per well, in 24-well plates.
Grow organoids for 11 days |
Fix recovered organoids (overnight in 4.0% paraformaldehyde), embedd them (2.0% Bacto-Agar: 2.5% gelatin for paraffin embedding) and subjected to histologic analyses. |
| Upstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
| Protocol tips |
Seed 2000 cells per well, in 24-well plates.
Grow organoids for 11 days |
| Downstream tips |
| Fix recovered organoids (overnight in 4.0% paraformaldehyde), embedd them (2.0% Bacto-Agar: 2.5% gelatin for paraffin embedding) and subjected to histologic analyses. |
| Upstream tips |
Protocol tips |
Downstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
Seed 2 × 104 cells/well of the flat bottom 24-well plates
Grow 3D organoids for 10–14 days |
Collect organoids for histological analysis |
| Upstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
| Protocol tips |
Seed 2 × 104 cells/well of the flat bottom 24-well plates
Grow 3D organoids for 10–14 days |
| Downstream tips |
| Collect organoids for histological analysis |
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