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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
| Use of sorted basal and luminal epithelial population |
Seed 5,000–20,000 cells per well for basal cells and 2,000–10,000 cells per well for luminal cells
Use of ultralow attachment 96-well plates
After 6 days, count the spheres with diameter greater than 30 μm |
|
| Upstream tips |
| Use of sorted basal and luminal epithelial population |
| Protocol tips |
Seed 5,000–20,000 cells per well for basal cells and 2,000–10,000 cells per well for luminal cells
Use of ultralow attachment 96-well plates
After 6 days, count the spheres with diameter greater than 30 μm |
| Upstream tips |
Protocol tips |
Downstream tips |
|
Seed of single primary mammary epithelial cells at limiting dilutions (100 cells/ml)
Use of 96-well ultra-low-attachment plates
Fix, stain and count colonies according to size and morphological criteria |
|
| Protocol tips |
Seed of single primary mammary epithelial cells at limiting dilutions (100 cells/ml)
Use of 96-well ultra-low-attachment plates
Fix, stain and count colonies according to size and morphological criteria |
| Upstream tips |
Protocol tips |
Downstream tips |
| To prevent attachment of cells all dishes/plates, coat them with polyhydroxyethylmethacrylate (PolyHEMA) |
Seed HMECs at a density of 10,000 cells/ml and 50,000 cells/ml, respectively, in 3-, 6-, or 10-cm cell culture dishes or 96-well flat-bottom plates |
|
| Upstream tips |
| To prevent attachment of cells all dishes/plates, coat them with polyhydroxyethylmethacrylate (PolyHEMA) |
| Protocol tips |
| Seed HMECs at a density of 10,000 cells/ml and 50,000 cells/ml, respectively, in 3-, 6-, or 10-cm cell culture dishes or 96-well flat-bottom plates |
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