3D Cell Culture Media Human primary breast ephitelial cells-Mammospheres

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Found 3 matching solutions for this experiment

MammoCult™ Human Medium Kit

STEMCELL technologies

Upstream tips
Use of sorted basal and luminal epithelial population
Protocol tips
Seed 5,000–20,000 cells per well for basal cells and 2,000–10,000 cells per well for luminal cells
Use of ultralow attachment 96-well plates
After 6 days, count the spheres with diameter greater than 30 μm
Protocol tips
Seed of single primary mammary epithelial cells at limiting dilutions (100 cells/ml)
Use of 96-well ultra-low-attachment plates
Fix, stain and count colonies according to size and morphological criteria
Upstream tips
To prevent attachment of cells all dishes/plates, coat them with polyhydroxyethylmethacrylate (PolyHEMA)
Protocol tips
Seed HMECs at a density of 10,000 cells/ml and 50,000 cells/ml, respectively, in 3-, 6-, or 10-cm cell culture dishes or 96-well flat-bottom plates
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