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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
| Defrost in advance the cultrex growth factor reduced BME type 2 gel on ice or in fridge |
If the cell pellet is red, lyse the erythrocytes
Prewarm 24-well suspension culture plates
Change medium every 4 days, passage of the organoids every 1-4 weeks
Culture every line of organoids physically separated to prevent cross-contamination
|
|
| Upstream tips |
| Defrost in advance the cultrex growth factor reduced BME type 2 gel on ice or in fridge |
| Protocol tips |
If the cell pellet is red, lyse the erythrocytes
Prewarm 24-well suspension culture plates
Change medium every 4 days, passage of the organoids every 1-4 weeks
Culture every line of organoids physically separated to prevent cross-contamination
|
| Upstream tips |
Protocol tips |
Downstream tips |
|
Use of collagen I gels
For detached gels, use siloxane-coated 24-well or 48-well plates
For attached gels, use uncoated 24-well plates
|
|
| Protocol tips |
Use of collagen I gels
For detached gels, use siloxane-coated 24-well or 48-well plates
For attached gels, use uncoated 24-well plates
|
| Upstream tips |
Protocol tips |
Downstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
Use of an eight-well LabTek chamber
Coat the wells with 60μL of a 60% Matrigel (BD Biosciences) solution in BBM |
|
| Upstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
| Protocol tips |
Use of an eight-well LabTek chamber
Coat the wells with 60μL of a 60% Matrigel (BD Biosciences) solution in BBM |
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