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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
| Use freshly sorted cells |
Seed 4,000 cells per well at a density of 8,000 viable cells/mL
Use low attachment plates
|
|
| Upstream tips |
| Use freshly sorted cells |
| Protocol tips |
Seed 4,000 cells per well at a density of 8,000 viable cells/mL
Use low attachment plates
|
| Upstream tips |
Protocol tips |
Downstream tips |
|
Seed 4 × 104 cells/ml to an ultra-low attachment tray |
|
| Protocol tips |
| Seed 4 × 104 cells/ml to an ultra-low attachment tray |
| Upstream tips |
Protocol tips |
Downstream tips |
|
Culture isolated MECs in MEC culture medium for 1 day, before mammosphere culture
Seed single MECs at a density of 25 000 viable cells/ml
Use of ultralow attachment plates
|
|
| Protocol tips |
Culture isolated MECs in MEC culture medium for 1 day, before mammosphere culture
Seed single MECs at a density of 25 000 viable cells/ml
Use of ultralow attachment plates
|
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