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Found 2 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
Use 24-well transwell filter inserts with 0.4um pore (Corning) in a 24-well tissue culture plate
Seed 2,000 cells for primary organoid (1°) formation
|
|
| Upstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
| Protocol tips |
Use 24-well transwell filter inserts with 0.4um pore (Corning) in a 24-well tissue culture plate
Seed 2,000 cells for primary organoid (1°) formation
|
| Upstream tips |
Protocol tips |
Downstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
Seed unsorted or sorted lung epithelial cells (1–2 × 104 cells in 50 μl Matrigel/well)
Change medium every 3 days.
|
|
| Upstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
| Protocol tips |
Seed unsorted or sorted lung epithelial cells (1–2 × 104 cells in 50 μl Matrigel/well)
Change medium every 3 days.
|
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