3D Cell Culture Media Mouse primary lung epithelial cells-organoids

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Found 2 matching solutions for this experiment

DMEM/F-12

Thermo Fisher Scientific

Upstream tips
Defrost the Matrigel in advance on ice or in fridge
Protocol tips
Use 24-well transwell filter inserts with 0.4um pore (Corning) in a 24-well tissue culture plate
Seed 2,000 cells for primary organoid (1°) formation
DMEM/F-12, no phenol red

Thermo Fisher Scientific

Upstream tips
Defrost the Matrigel in advance on ice or in fridge
Protocol tips
Seed unsorted or sorted lung epithelial cells (1–2 × 104 cells in 50 μl Matrigel/well)
Change medium every 3 days.
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