3D Cell Culture Media Mouse primary mammary ephitelial cells- organoids

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Found 3 matching solutions for this experiment

DMEM/F-12

Thermo Fisher Scientific

Upstream tips
Defrost the Matrigel in advance on ice or in fridge
Protocol tips
Keep isolated organoids on ice up to 2 h before seeding for 3D culture
Seed 200, 400, or 1000 organoids per dome
Use of 24-well culture plate
Downstream tips
Collect organoids for histology, gene expression, and Western blot analysis
Gibco™Advanced DMEM/F-12

Thermo Fisher Scientific

Upstream tips
Defrost in advance basement membrane extract (BME; Cultrex) on ice or in fridge
Protocol tips
Use uncoated 24-well plates
Refresh medium every 2-3 days
Gibco DMEM/F-12, HEPES

Thermo Fisher Scientific

Upstream tips
Defrost the Matrigel in advance on ice or in fridge
Protocol tips
Use of an eight-well LabTek chamber
Coat the wells with 60μL of a 60% Matrigel (BD Biosciences) solution in BBM
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