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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
Optional: co-culture of mouse intestinal epithelial cells and isolated fibroblasts |
|
| Upstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
| Protocol tips |
| Optional: co-culture of mouse intestinal epithelial cells and isolated fibroblasts |
| Upstream tips |
Protocol tips |
Downstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
After 2 days in culture remove CHIR and Rock inhibitor
|
|
| Upstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
| Protocol tips |
After 2 days in culture remove CHIR and Rock inhibitor
|
| Upstream tips |
Protocol tips |
Downstream tips |
| Defrost the growth factor reduced Matrigel in advance, on ice or in fridge |
Seed different volumes of organoid units to assess survival rate and growth |
|
| Upstream tips |
| Defrost the growth factor reduced Matrigel in advance, on ice or in fridge |
| Protocol tips |
| Seed different volumes of organoid units to assess survival rate and growth |
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