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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
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Seed cancer cells at a density of 1000cells/ml
Use of ultra-low adhesion plates
Count mammospheres with diameter > 75μm |
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| Protocol tips |
Seed cancer cells at a density of 1000cells/ml
Use of ultra-low adhesion plates
Count mammospheres with diameter > 75μm |
| Upstream tips |
Protocol tips |
Downstream tips |
|
Incubation of mammospheres under hypoxia
Passages of mammospheres every 4 days
Count of mammospheres at least 50 μm diameter
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| Protocol tips |
Incubation of mammospheres under hypoxia
Passages of mammospheres every 4 days
Count of mammospheres at least 50 μm diameter
|
| Upstream tips |
Protocol tips |
Downstream tips |
| Process tumor specimens 1h of surgery |
Use ultralow attachment plates
Change media every third day |
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| Upstream tips |
| Process tumor specimens 1h of surgery |
| Protocol tips |
Use ultralow attachment plates
Change media every third day |
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