Angiogenesis assay human - bone marrow mononuclear cells

A standard angiogenic assay involves the autonomous endothelial cell response of self-organization into microvessels, also known as tubes when seeded on a basement membrane matrix in the presence of the appropriate growth factors. However, the component of basement membrane matrix may also affect the tube formation by endothelial cells. Hence it is important to use a standard angiogenesis assay kit or use the same membrane matrix with known composition to standardize the assay conditions.

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5 years ago

5 years ago by Sander J. Mikkelsen Denmark

What is the difference between endothelial cells and HUVEC cells for angiogenesis assay?

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In Vitro Angiogenesis Assay Kit

Merck Millipore

Upstream tips	Protocol tips	Downstream tips
	- Supplemented with 1% fetal bovine serum and with or without 50 ng/ml vascular endothelial growth factor (VEGF) as chemo-attractant and incubated at 37°C for 24 hours.

Protocol tips
- Supplemented with 1% fetal bovine serum and with or without 50 ng/ml vascular endothelial growth factor (VEGF) as chemo-attractant and incubated at 37°C for 24 hours.

Manufacturer protocol Publication protocol 1 Relevant paper

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