Apoptosis assay cell type - A2780

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

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Found 3 matching solutions for this experiment

Annexin V-FITC Apoptosis Detection Kit

Sigma-Aldrich

Upstream tips	Protocol tips	Downstream tips
- Prepare 1x Binding Buffer by diluting 1 ml of the 10x Binding Buffer with 9 ml of deionized water. - Dissolve the staurosporine in DMSO to a concentration of 100 mg/ml	- 1 × 10^4 cells were collected and stained with Annexin V-FITC

Upstream tips
- Prepare 1x Binding Buffer by diluting 1 ml of the 10x Binding Buffer with 9 ml of deionized water. - Dissolve the staurosporine in DMSO to a concentration of 100 mg/ml
Protocol tips
- 1 × 10^4 cells were collected and stained with Annexin V-FITC

Manufacturer protocol Publication protocol 1 Relevant paper

single stranded-DNA Apoptosis ELISA kit

Millipore

Upstream tips	Protocol tips	Downstream tips
- 1×10^4 cells/ml were used for the assay. - Treated with 25 µM myricetin for 48 h at 37°C

Upstream tips
- 1×10^4 cells/ml were used for the assay. - Treated with 25 µM myricetin for 48 h at 37°C

Manufacturer protocol Publication protocol 1 Relevant paper

PE Annexin V Apoptosis Detection Kit I

BD Biosciences

Upstream tips	Protocol tips	Downstream tips
	Add 5 µl of PE Annexin V and 5 µl 7-AAD

Protocol tips
Add 5 µl of PE Annexin V and 5 µl 7-AAD

Manufacturer protocol Publication protocol 1 Relevant paper

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