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|- Do not warm the stock reagents before dispensing them from containers.
- Ethanol should not be substituted for n-butanol as it will destain nuclei.
|- If staining of both nuclei and cytoplasm is apparent, the nonspecific component often can be mitigated by reducing the development time in substrate.
- If a very high percentage of the nuclei in a tissue can be stained with TdT,
but not without TdT, there appears to be staining of DNA only.