Apoptosis assay cell type - K562

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

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Found 3 matching solutions for this experiment

Upstream tips
- 1 × 105 cells/mL were cultured.

- Treated with various concentrations of M. integrifolia extract for 24, 48 and 72 h at 37 °C.
Protocol tips
- After adding Annexin V–FITC and PI they were incubated for 15 min
Upstream tips
- 5×10^5 cells were seeded.

- Pretreated with 0.3 µM imatinib for 24 h
Protocol tips
- Treated with FITC Annexin V and PI and incubated for 15 min at RT (25°C) in the dark
Protocol tips
- Always stain cells with Annexin before fixation (makes cell membrane leaky)
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