Apoptosis assay cell type - MCF-7 human breast cancer

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

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Found 2 matching solutions for this experiment

Annexin V / 7-AAD

Beckman Coulter

Upstream tips
- Cells were exposed to 250, 500 and 1000 µM of compound 3a and 10, 25 or 50 µM of 3b and 4 for 24 h
Downstream tips
- The incubation with Annexin A5-FITC and 7-AAD should be carried out on ice so as to arrest further progress
of the cells through the stages of viability > apoptosis > secondary necrosis.
Upstream tips
- 1.3 × 10^5 cells were seeded and incubated with DCM-DS (12.5-50 μg/mL) for 24 h
Protocol tips
- Annexin V-FITC is treated on cells and incubated for 10 min at room temperature in dark
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