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Found 2 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
| - Cells were exposed to 250, 500 and 1000 µM of compound 3a and 10, 25 or 50 µM of 3b and 4 for 24 h |
|
- The incubation with Annexin A5-FITC and 7-AAD should be carried out on ice so as to arrest further progress
of the cells through the stages of viability > apoptosis > secondary necrosis. |
| Upstream tips |
| - Cells were exposed to 250, 500 and 1000 µM of compound 3a and 10, 25 or 50 µM of 3b and 4 for 24 h |
| Downstream tips |
- The incubation with Annexin A5-FITC and 7-AAD should be carried out on ice so as to arrest further progress
of the cells through the stages of viability > apoptosis > secondary necrosis. |
| Upstream tips |
Protocol tips |
Downstream tips |
| - 1.3 × 10^5 cells were seeded and incubated with DCM-DS (12.5-50 μg/mL) for 24 h |
- Annexin V-FITC is treated on cells and incubated for 10 min at room temperature in dark |
|
| Upstream tips |
| - 1.3 × 10^5 cells were seeded and incubated with DCM-DS (12.5-50 μg/mL) for 24 h |
| Protocol tips |
| - Annexin V-FITC is treated on cells and incubated for 10 min at room temperature in dark |
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