Apoptosis assay cell type - MCF10A

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

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Found 1 matching solution for this experiment

eBioscience™ Annexin V-FITC Apoptosis Detection Kit

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
- 1.3 × 10^5 cells were seeded and incubated with DCM-DS (12.5-50 μg/mL) for 24 h	- Annexin V-FITC is treated on cells and incubated for 10 min at room temperature in dark

Upstream tips
- 1.3 × 10^5 cells were seeded and incubated with DCM-DS (12.5-50 μg/mL) for 24 h
Protocol tips
- Annexin V-FITC is treated on cells and incubated for 10 min at room temperature in dark

Manufacturer protocol Publication protocol 1 Relevant paper

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