Apoptosis assay cell type - MCF10A

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

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Upstream tips
- 1.3 × 10^5 cells were seeded and incubated with DCM-DS (12.5-50 μg/mL) for 24 h
Protocol tips
- Annexin V-FITC is treated on cells and incubated for 10 min at room temperature in dark
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