Apoptosis assay cell type - OVCAR-3

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

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Found 3 matching solutions for this experiment

Annexin V-FITC Kit

Miltenyibiotec

Upstream tips	Protocol tips	Downstream tips
	After incubation for 48 h, the cells were washed with PBS, trypinsized, and stained with fluorescein isothycanateconjugated annexin V using Annexin V-FITC kit (Miltenyi Biotec, Germany) and propidium iodide (PI), following the manufacturer’s protocol.

Protocol tips
After incubation for 48 h, the cells were washed with PBS, trypinsized, and stained with fluorescein isothycanateconjugated annexin V using Annexin V-FITC kit (Miltenyi Biotec, Germany) and propidium iodide (PI), following the manufacturer’s protocol.

Manufacturer protocol Publication protocol 1 Relevant paper

bisBenzimide H 33342 trihydrochloride

Sigma-Aldrich

Upstream tips	Protocol tips	Downstream tips
- 1 × 10^5 cells/well were seeded.	- Cells were treated with various concentrations (0–4 μM) of ChK for 24 h. - Hoechst 33342 in PBS for 10 min in the dark at 37 °C.

Upstream tips
- 1 × 10^5 cells/well were seeded.
Protocol tips
- Cells were treated with various concentrations (0–4 μM) of ChK for 24 h. - Hoechst 33342 in PBS for 10 min in the dark at 37 °C.

Publication protocol 1 Relevant paper

single stranded-DNA Apoptosis ELISA kit

Millipore

Upstream tips	Protocol tips	Downstream tips
- 1×10^4 cells/ml were used for the assay. - Treated with 25 µM myricetin for 48 h at 37°C

Upstream tips
- 1×10^4 cells/ml were used for the assay. - Treated with 25 µM myricetin for 48 h at 37°C

Manufacturer protocol Publication protocol 1 Relevant paper

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