Apoptosis assay cell type - PA-1 human ovarian cancer

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

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Found 2 matching solutions for this experiment

Protocol tips
Add 5 ml of Annexin V FITC Conjugate and 10 ml of
Propidium Iodide Solution to each cell suspension and incubate the tubes at room temperature for exactly
10 minutes and protect from light.
Protocol tips
Add 5 µl of PE Annexin V and 5 µl 7-AAD. and incubate for 15 min at RT (25°C) in the dark.
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