Apoptosis assay cell type - PANC-1

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

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Found 4 matching solutions for this experiment

ApoAlert™ Annexin V-FITC Apoptosis Kit

Takara Bio Inc

Upstream tips	Protocol tips	Downstream tips
- 0.5-1 × 10^6 cells were cultured in a 6 welll plate - cells were pretreated with absence or presence of AZD8055 (500 nM). - Radiation was applied 4 h later.	- Annexin V and PI are added and incubated for 15 min at RT in the dark

Upstream tips
- 0.5-1 × 10^6 cells were cultured in a 6 welll plate - cells were pretreated with absence or presence of AZD8055 (500 nM). - Radiation was applied 4 h later.
Protocol tips
- Annexin V and PI are added and incubated for 15 min at RT in the dark

Manufacturer protocol Publication protocol 1 Relevant paper

FITC Annexin V Apoptosis Detection Kit with 7-AAD

BioLegend

Upstream tips	Protocol tips	Downstream tips
- Cells were pre treated with 8 μM sorafenib for 24h. - 1 × 10^6 cells were used for the assay.	- Add FITC Annexin V and 7-AAD Viability Staining Solution and incubate for 15 min at RT.

Upstream tips
- Cells were pre treated with 8 μM sorafenib for 24h. - 1 × 10^6 cells were used for the assay.
Protocol tips
- Add FITC Annexin V and 7-AAD Viability Staining Solution and incubate for 15 min at RT.

Manufacturer protocol Publication protocol 1 Relevant paper

FITC Annexin V Apoptosis Detection Kit I (RUO)

BD Biosciences

Upstream tips	Protocol tips	Downstream tips
- Cells were treated with 1 μM 3-Cl-AHPC and AHP3 for various indicated time.	- Treated with FITC Annexin V and PI and incubated for 15 min at RT (25°C) in the dark

Upstream tips
- Cells were treated with 1 μM 3-Cl-AHPC and AHP3 for various indicated time.
Protocol tips
- Treated with FITC Annexin V and PI and incubated for 15 min at RT (25°C) in the dark

Manufacturer protocol Publication protocol 1 Relevant paper

APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	- Cells were incubated with ice-cold 70% ethanol overnight at −20 °C. - Cells were incubated for 1 h at 36 °C after treated with DNA-labeling solution

Protocol tips
- Cells were incubated with ice-cold 70% ethanol overnight at −20 °C. - Cells were incubated for 1 h at 36 °C after treated with DNA-labeling solution

Manufacturer protocol Publication protocol 1 Relevant paper

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