Apoptosis assay cell type - SKOV3 human ovarian carcinoma

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

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Found 3 matching solutions for this experiment

Upstream tips
- 3 × 10^4 were seeded in 96 well plate.
Protocol tips
- Cells were cultured in culture medium with and without fresh medium containing 5% FBS (as control) or 5% ascite in presence or not of H2O2 for 6 hours before adding APOPercentage Dye.
Downstream tips
- Wells were shaken for 20 minutes before being read at 540 nm
Upstream tips
- Cells were pre treated with artonin E at different time points
Protocol tips
- Cells were Dounce-homogenized before centrifuging at 7,000×g for 10 min at 4°C.
Upstream tips
- Do not freeze components
Protocol tips
- Add FITC Annexin V and 7-AAD Viability Staining Solution and incubate for 15 min at RT.
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