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Found 5 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
| - Cells were treated with LfcinB-P13 (range, 0–60 µg/ml) for 24 h. |
- Treated with AV, PI and incubate 10-15 minutes at room temperature. |
- Due to the calcium dependence of the Annexin V:PS interaction, it is critical to avoid buffers containing EDTA or other calcium chelators during
Annexin V experiments.
- Annexin V can only be used as a marker of apoptosis in cells where the plasma membrane is intact because destroying the
integrity of the plasma membrane will allow non-specific binding of Annexin V to PS inside the cell. |
| Upstream tips |
| - Cells were treated with LfcinB-P13 (range, 0–60 µg/ml) for 24 h. |
| Protocol tips |
| - Treated with AV, PI and incubate 10-15 minutes at room temperature. |
| Downstream tips |
- Due to the calcium dependence of the Annexin V:PS interaction, it is critical to avoid buffers containing EDTA or other calcium chelators during
Annexin V experiments.
- Annexin V can only be used as a marker of apoptosis in cells where the plasma membrane is intact because destroying the
integrity of the plasma membrane will allow non-specific binding of Annexin V to PS inside the cell. |
| Upstream tips |
Protocol tips |
Downstream tips |
|
The cell apoptosis was measured by flow cytometry
analysis with an Annexin-V/FITC and propidium iodide
(PI) apoptosis detection kit (BioVision, USA), according to the manufacturer’s standards. Briefly, after 48 h
of transfection, the cells were collected and suspended
in annexin-binding buffer and exposed to Annexin-VFITC and PI for 15 min in the dark at room temperature. The apoptotic percentage of cells was quantified by
flow cytometry |
|
| Protocol tips |
The cell apoptosis was measured by flow cytometry
analysis with an Annexin-V/FITC and propidium iodide
(PI) apoptosis detection kit (BioVision, USA), according to the manufacturer’s standards. Briefly, after 48 h
of transfection, the cells were collected and suspended
in annexin-binding buffer and exposed to Annexin-VFITC and PI for 15 min in the dark at room temperature. The apoptotic percentage of cells was quantified by
flow cytometry |
| Upstream tips |
Protocol tips |
Downstream tips |
- Dissolve the staurosporine in DMSO to a concentration
of 100 mg/ml..
- 1 × 10^6 cells/well were cultured in 25 cm2 culture flask for 24h |
|
|
| Upstream tips |
- Dissolve the staurosporine in DMSO to a concentration
of 100 mg/ml..
- 1 × 10^6 cells/well were cultured in 25 cm2 culture flask for 24h |
| Upstream tips |
Protocol tips |
Downstream tips |
|
- 48h post transfection, cells were treated with Annexin-VFITC and PI for 15 min in the dark at room temperature. |
|
| Protocol tips |
| - 48h post transfection, cells were treated with Annexin-VFITC and PI for 15 min in the dark at room temperature. |
| Upstream tips |
Protocol tips |
Downstream tips |
|
- Treated with FITC Annexin V and PI and incubated for 15 min at RT (25°C) in the dark |
|
| Protocol tips |
| - Treated with FITC Annexin V and PI and incubated for 15 min at RT (25°C) in the dark |
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