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|- Cells should be healthy and not overcrowded as results of the experiments will depend significantly on the cells’ condition.|
|- Incase of Low CYTO-ID Green
dye staining in all treatments, increase the Increase the reagent concentration (500X dilution of the dye is
recommended) and increase the incubation time.
- Incase of High CYTO-ID Green staining, it could be that the cell culture medium was depleted of nutrients and so change media 4-8 hours before the experiment.