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Found 1 matching solution for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
| This kit uses a green fluorescent probe that reacts with autophagic vacuoles and is detectable by flow cytometry |
|
Incase of Low CYTO-ID Green dye staining in all treatments, increase the reagent concentration (500X dilution of the dye is recommended) and the incubation time.
Incase of High CYTO-ID Green staining, it could be that the cell culture medium was depleted of nutrients and so change media 4-8 hours before the experiment |
| Upstream tips |
| This kit uses a green fluorescent probe that reacts with autophagic vacuoles and is detectable by flow cytometry |
| Downstream tips |
Incase of Low CYTO-ID Green dye staining in all treatments, increase the reagent concentration (500X dilution of the dye is recommended) and the incubation time.
Incase of High CYTO-ID Green staining, it could be that the cell culture medium was depleted of nutrients and so change media 4-8 hours before the experiment |
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