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|This kit uses a green fluorescent probe that reacts with autophagic vacuoles and is detectable by flow cytometry|
|Incubate cells for 30 minutes at 37°C in the dark, wash with 1× assay buffer, and resuspend in 500 μL fresh 1× assay buffer. Subject the cells to flow cytometric analysis using the green (FL1) channel.|
|Incase of Low CYTO-ID Green dye staining in all treatments, increase the reagent concentration (500X dilution of the dye is recommended) and the incubation time.
Incase of High CYTO-ID Green staining, it could be that the cell culture medium was depleted of nutrients and so change media 4-8 hours before the experiment