No discussions found
Found 1 matching solution for this experiment
|Use Cyto-ID dye (1 μl/4 ml assay buffer) for 30 minutes in dark at RT.|
|Incase of Low CYTO-ID dye staining in all treatments, increase the reagent concentration (500X dilution of the dye is recommended) and the incubation time.
Incase of High CYTO-ID staining, it could be that the cell culture medium was depleted of nutrients and so change media 4-8 hours before the experiment