Autophagy assay cell type - T98

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

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Found 1 matching solution for this experiment

The Premo Autophagy Tandem Sensor RFP-GFP-LC3B Kit

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
For optimal results you may alter the PPC, cell density, temperature, and incubation time.	Infect the cells with 60ppc (particle per cell) for 24hour.

Upstream tips
For optimal results you may alter the PPC, cell density, temperature, and incubation time.
Protocol tips
Infect the cells with 60ppc (particle per cell) for 24hour.

Manufacturer protocol Publication protocol 1 Relevant paper

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