Bacterial cell culture media Enterococcus faecium

Bacterial culture is a process of letting bacteria multiply in a controlled fashion (temperature, humidity, oxygen content or shaking), in a predetermined culture medium (antibiotic resistance to obtain homogenous clones). It is an important step, especially during cloning, as a single cell can be grown homogeneously (on semi-solid or in liquid conditions) to obtain colonies. As mentioned, bacteria can be cultured in broth cultures (Luria broth or LB) or Petri dishes (Agar plates). A specific antibiotic can be added to the broth or agar plates in order to grow bacteria which have the gene insert conferring its resistance to that antibiotic. Following points are necessary to consider for optimal growth conditions: 1. In general, most bacteria grow well at 37C, but there are some strains which require growth temperatures between 25-30C. 2. It is ideal in broth cultures to fill the flask to ⅓ or less of the total flask volume for optimal aerobic growth. 3. Shaking speeds between 140-180 rpm are appropriate to ensure aeration and that the cells are surrounded by fresh media, and do not settle.

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INTRAY® COLOREX™ VRE - PREPARED PLATED CULTURE MEDIA FOR VANCOMYCIN RESISTANT ENTEROCOCCI

BIOMED

Upstream tips	Protocol tips	Downstream tips
	Of the 400 specimens tested, four were excluded from the analysis because they grew only VRE Enterococcus raffinosus, an organism for which none of the chromogenic agars have FDA approval. Of the 396 remaining specimens, 297 (75%) were negative for VRE. The performance characteristics of each medium, BEAV agar, and BEAV broth were compared using combined data (Table 2). Overall, the sensitivities of all of the chromogenic media (89.9 to 94.9%) were higher than that of the BEAV agar (84.8%). chromID had the highest sensitivity (94.9%). All of the chromogenic media recovered significantly more VRE than did the BEAV agar. There was no statistical difference in VRE detection performance among the five chromogenic media

Protocol tips
Of the 400 specimens tested, four were excluded from the analysis because they grew only VRE Enterococcus raffinosus, an organism for which none of the chromogenic agars have FDA approval. Of the 396 remaining specimens, 297 (75%) were negative for VRE. The performance characteristics of each medium, BEAV agar, and BEAV broth were compared using combined data (Table 2). Overall, the sensitivities of all of the chromogenic media (89.9 to 94.9%) were higher than that of the BEAV agar (84.8%). chromID had the highest sensitivity (94.9%). All of the chromogenic media recovered significantly more VRE than did the BEAV agar. There was no statistical difference in VRE detection performance among the five chromogenic media

Protocol tips

Of the 400 specimens tested, four were excluded from the analysis because they grew only VRE Enterococcus raffinosus, an organism for which none of the chromogenic agars have FDA approval. Of the 396 remaining specimens, 297 (75%) were negative for VRE. The performance characteristics of each medium, BEAV agar, and BEAV broth were compared using combined data (Table 2). Overall, the sensitivities of all of the chromogenic media (89.9 to 94.9%) were higher than that of the BEAV agar (84.8%). chromID had the highest sensitivity (94.9%). All of the chromogenic media recovered significantly more VRE than did the BEAV agar. There was no statistical difference in VRE detection performance among the five chromogenic media

Manufacturer protocol Publication protocol 1 Relevant paper

VRESelect Medium #63751

Bio-Rad Laboratories

Upstream tips	Protocol tips	Downstream tips
	Of the 400 specimens tested, four were excluded from the analysis because they grew only VRE Enterococcus raffinosus, an organism for which none of the chromogenic agars have FDA approval. Of the 396 remaining specimens, 297 (75%) were negative for VRE. The performance characteristics of each medium, BEAV agar, and BEAV broth were compared using combined data (Table 2). Overall, the sensitivities of all of the chromogenic media (89.9 to 94.9%) were higher than that of the BEAV agar (84.8%). chromID had the highest sensitivity (94.9%). All of the chromogenic media recovered significantly more VRE than did the BEAV agar. There was no statistical difference in VRE detection performance among the five chromogenic media

Protocol tips
Of the 400 specimens tested, four were excluded from the analysis because they grew only VRE Enterococcus raffinosus, an organism for which none of the chromogenic agars have FDA approval. Of the 396 remaining specimens, 297 (75%) were negative for VRE. The performance characteristics of each medium, BEAV agar, and BEAV broth were compared using combined data (Table 2). Overall, the sensitivities of all of the chromogenic media (89.9 to 94.9%) were higher than that of the BEAV agar (84.8%). chromID had the highest sensitivity (94.9%). All of the chromogenic media recovered significantly more VRE than did the BEAV agar. There was no statistical difference in VRE detection performance among the five chromogenic media

Protocol tips

Manufacturer protocol Publication protocol 1 Relevant paper

CHROMID® VRE

Biomerieux

Upstream tips	Protocol tips	Downstream tips
	-Incubation : 48 hours

Protocol tips
-Incubation : 48 hours

Manufacturer protocol Publication protocol 1 Relevant paper

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