Bacterial cell culture media Salmonella enterica

Bacterial culture is a process of letting bacteria multiply in a controlled fashion (temperature, humidity, oxygen content or shaking), in a predetermined culture medium (antibiotic resistance to obtain homogenous clones). It is an important step, especially during cloning, as a single cell can be grown homogeneously (on semi-solid or in liquid conditions) to obtain colonies. As mentioned, bacteria can be cultured in broth cultures (Luria broth or LB) or Petri dishes (Agar plates). A specific antibiotic can be added to the broth or agar plates in order to grow bacteria which have the gene insert conferring its resistance to that antibiotic. Following points are necessary to consider for optimal growth conditions: 1. In general, most bacteria grow well at 37C, but there are some strains which require growth temperatures between 25-30C. 2. It is ideal in broth cultures to fill the flask to ⅓ or less of the total flask volume for optimal aerobic growth. 3. Shaking speeds between 140-180 rpm are appropriate to ensure aeration and that the cells are surrounded by fresh media, and do not settle.

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Found 3 matching solutions for this experiment

BRAIN HEART INFUSION BROTH

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
-Store the dehydrated medium at 10-30°C and use before the expiry date on the label. Store tubed or bottled medium in the dark and below 20°C.	-Tubes of Oxoid Brain Heart Infusion Broth which are not used the same day as sterilized should be placed in a boiling water bath for several minutes to remove absorbed oxygen, and cooled rapidly without shaking, just before use.

Upstream tips
-Store the dehydrated medium at 10-30°C and use before the expiry date on the label. Store tubed or bottled medium in the dark and below 20°C.
Protocol tips
-Tubes of Oxoid Brain Heart Infusion Broth which are not used the same day as sterilized should be placed in a boiling water bath for several minutes to remove absorbed oxygen, and cooled rapidly without shaking, just before use.

Manufacturer protocol Publication protocol 1 Relevant paper

Neogen-Brain-Heart Infusion Broth

Neogen

Upstream tips	Protocol tips	Downstream tips
-Store dehydrated culture media at 2 – 30°C away from direct sunlight. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect from moisture and light by keeping container tightly closed.	-The medium is suitable for use as a blood culture medium or as an enrichment broth for fastidious organisms.

Upstream tips
-Store dehydrated culture media at 2 – 30°C away from direct sunlight. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect from moisture and light by keeping container tightly closed.
Protocol tips
-The medium is suitable for use as a blood culture medium or as an enrichment broth for fastidious organisms.

Manufacturer protocol Publication protocol 1 Relevant paper

BHI - Brain Heart Infusion

HiMEDIA

Upstream tips	Protocol tips	Downstream tips
-Store between 15-25°C.	- With the addition of 10% defibrinated sheep blood, it is useful for isolation and cultivation of Histoplasma capsulatum and other fungi. For selective isolation of fungi, addition of gentamicin and/or chloramphenicol is recommended.

Upstream tips
-Store between 15-25°C.
Protocol tips
- With the addition of 10% defibrinated sheep blood, it is useful for isolation and cultivation of Histoplasma capsulatum and other fungi. For selective isolation of fungi, addition of gentamicin and/or chloramphenicol is recommended.

Manufacturer protocol Publication protocol 1 Relevant paper

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